虞沂

职务/职称：教授、博导、珞珈青年学者

通讯地址：湖北省武汉市武昌区东湖路185号金沙集团wwW3354CC，430071

E-mail: yu_yi@whu.edu.cn

电话: 027-68752491

学习简历

1996.9 – 2002.6   华中农业大学生物技术基地班，本硕连读

2003.9 – 2007.6  中科院微生物研究所，博士

工作简历

2007.7 – 2009.6  上海交通大学生命科学技术学院生物工程系，讲师

2007.3 – 2009.6  上海有机化学研究所，合作研究

2009.7 – 2010.6  华中农业大学生命科学技术学院，讲师

2012.8 – 2013.8  美国Washington University in Saint Louis, 访问学者

2010.7 至今        金沙集团wwW3354CC，副教授、教授、博导

2018.1 – 2018.12 英国牛津大学，高级访问学者

获奖情况

教育部2007年度高等学校科学技术奖自然科学奖二等奖

2015-2016年度武汉大学医学部突出贡献奖

2016-2017 年度武汉大学医学部朱裕璧奖

2016年度武汉大学十佳教师（第十届）

第八届湖北省高等学校教学成果奖三等奖

2016年世界大学生药苑论坛优秀壁报奖、优秀论文奖，以及创新成果一等奖

研究领域

天然产物的组合生物合成与应用

研究兴趣与方向

以中草药和微生物来源天然产物为基础，研制和开发新药一直是国际化学界和医药界共同关注的重点领域。天然产物的生物合成是以小分子的简单化合物作为底物，经过一系列顺序催化反应，最终形成复杂化学结构的过程。本人的研究兴趣在于通过代谢工程和组合生物合成研究植物和微生物来源的复杂天然产物的生物合成，即利用基因工程技术，有目的地对细胞代谢途径进行精确的修饰或改造，以提高目的天然产物活性或产量、或合成新的代谢产物；或是通过操纵天然产物生物合成途径中的基因来产生非天然的天然产物类似物库，并从中发现和发展更具应用价值的药物。

承担课题与经费

2010年度武汉市青年科技晨光计划，6万

2010年度教育部新教师基金，3万

2011年国家自然科学基金青年项目，25万

2011年973子课题，26万

2014年973子课题，28.8万

2015年国家自然科学基金面上项目，78万

2017年武汉大学自主科研-拔尖创新人才，15万

2018年国家自然科学基金中英合作交流项目，10万

2018年国家自然科学基金面上项目，65万

2019年国家重点研发计划合成生物学重点专项子课题，80万

教学情况

本科生课程：免疫学，生物化学

研究组成员

博士后2名，博士研究生2名，硕士研究生7名

科研成果:（*通讯作者）

1. Haigang Song, Cong Rao, Zixin Deng, Yi Yu*, James Naismith*. The biosynthetic pathway of the benzoxazole in nataxazole proceeds via an unstable ester and has synthetic utility. Angew Chem Int Ed Engl., 2020, DOI: 10.1002/ange.201915685.
   

2. Lie Ma#, Siyao Sun#, Ziyu Yuan, Zixin Deng, Yi Yu*. Three putative DNA replication/repair elements encoding genes confer self-resistance to distamycin in Streptomyces netropsis. Acta Biochimica et Biophysica Sinica. 2019,10.1093/abbs/gmz133.

3. Yating Liu#, Li Su#, Qing Fang, Jioji Tabudravu, Xiaohui Yang, Kirstie Rickaby, Laurent Trembleau, Kwaku Kyeremeh, Zixin Deng, Hai Deng*, Yi Yu*. Enzymatic reconstitution and biosynthetic investigation of the bacterial carbazole neocarazostatin A. Journal of Organic Chemistry, 2019, DOI: 10.1039/c9qo01248f.

4. Yingying Chang, Baozhong Chai, Yunkun Ding, Min He, Linghui Zheng, Yun Teng, Zixin Deng, Yi Yu*, Tiangang Liu*. Overproduction of gentamicin B in industrial strain Micromonospora echinospora CCTCC M 2018898 by cloning of the missing genes genR and genS. Metabolic Engineering Communications, 2019, 9, e00096.

5. Yun X, Zhang Q, Lv M, Deng H, Deng Z, Yu Y.* In vitro reconstitution of the biosynthetic pathway of 3-hydroxypicolinic acid. Organic & Biomolecular Chemistry, 2019, 17(3):454-460.
   

   
   

6. Ding W., Ji W., Wu Y., Wu R., Liu W., Ma X., Zhang W., Xu P., Deng Z., Tang B., Yu Y.*, Zhang Q*. Biosynthesis of the nosiheptide indole side ring centers on a cryptic carrier protein NosJ. Nature Communications, 2017, 8(1):437. DOI: 10.1038/s41467-017-00439-1.

7. Yu Y., Zhang Q., Deng Z., Parallel pathways in the biosynthesis of aminoglycoside antibiotics. F1000Res. 2017, doi: 10.12688/ f1000research.11104.1.

8. Su Li, Zhang R, Kyeremeh K, Deng Z, Deng H* and Yu Y*. Dissection of the neocarazostatin A C4 alkyl side chain biosynthesis by in vitro reconstitution. Organic & Biomolecular Chemistry, 2017, 15(18): 3843-3848

9. Ding W, Li Y, Zhao J, Ji X, Mo T, Qianzhu H, Tu T, Deng Z, Yu Y, Chen F, Zhang Q*. The Catalytic Mechanism of the Class C Radical S-Adenosylmethionine Methyltransferase NosN. Angew Chem Int Ed Engl., 2017, 56(14):3857-3861.

10. Ding W, Wu Y, Ji X, Qianzhu H, Chen F, Deng Z, Yu Y, Zhang Q*. Nucleoside-linked shunt products in the reaction catalyzed by the class C radical S-adenosylmethionine methyltransferase NosN. Chem Commun (Camb)., 2017, 53(37):5235-5238.

11. Su Li, Lv Meinan, Kyeremeh Kwaku, Deng Zixin, Deng Hai and Yu Yi *. A ThDP-dependent enzymatic carboligation reaction involved in Neocarazostatin A tricyclic carbazole formation. Organic & Biomolecular Chemistry, 2016, 14(37): 8679-8684.

12. Lv M, Ji X, Zhao J, Li Y, Zhang C, Su L, Ding W, Deng Z, Yu Yi* and Zhang Q*. Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis. J AM CHEM SOC, 2016, 138(20):6427-35.

13. Huang S., Elsayed S., Lv M., Tabudravu J., Rateb M., Gyampoh R., Kyeremyh K., Ebel R., Jaspars M., Deng Z., Yu Yi* and Deng H.* Biosynthesis of neocarazostatin A reveals the sequential carbazole prenylation and hydroxylation in the tailoring steps. Chemistry & Biology, 2015, 22(12):1633-42.

14. Lv Meinan, Zhao Junfeng, Deng Zixin and Yu Yi*. Characterization of the biosynthetic gene cluster for benzoxazole antibiotics A33853 reveals unusual assembly logic. Chemistry & Biology, 2015, 22(10):1313-1324.

15. Huang S, Tabudravu J, Elsayed SS, Travert J, Peace D, Tong MH, Kyeremeh K, Kelly SM, Trembleau L, Ebel R, Jaspars M, Yu Yi* and Deng H*. Discovery of a Single Monooxygenase that Catalyzes Carbamate Formation and Ring Contraction in the Biosynthesis of the Legonmycins. Angew Chem Int Ed Engl. 2015, 54(43):12697-12701.

16. Huang S, Tong MH, Qin Z, Deng Z, Deng H, Yu Y.*. Identification and characterization of the biosynthetic gene cluster of thiolutin, a tumor angiogenesis inhibitor, in Saccharothrix algeriensis NRRL B-24137. Anticancer Agents Med Chem, 2015, 15(3):277-84.

17. Liu G, Zhang M, Mo T, He L, Zhang W, Yu Y*, Zhang Q*, Ding W*. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus. Biochem Biophys Res Commun., 2015, 467(4):1058-62.

18. Long Ma, Axel Bartholome, Ming Him Tong, Zhiwei Qin, Yi Yu, Thomas Shepherd, Kwaku Kyeremeh, Hai Deng* and David O'Hagan*. Identification of a fluorometabolite from Streptomyces sp. MA37: (2R3S4S)-5-fluoro-2,3,4-tri-hydroxypentanoic acid. Chemical Science, 2015, 6(2):1414-1419.

19. Hao C.L., Huang S., Deng Z.X., Zhao C.M., Yu Y.* Mining of the pyrrolamide antibiotics analogs in Streptomyces netropsis reveals the amidohydrolase-dependent "iterative strategy" underlying the pyrrole polymerization. PLOS ONE, 2014, 9(6):e99077.

20. Qin Zhiwei, Wang Xiaoling, Rateb Mostafa, Ass'ad Lina, Jaspars Marcel, Deng Zixin, Yu Yi, Deng Hai*. Disruption of a Methyltransferase Gene in Actinomycin G Gene Cluster in Streptomyces iakyrus Increases the Production of Phenazinomycin. FEMS Microbiology Letters, 2014, 352(1): 62-8.

21. Sheng Huang, Long Ma, Ming Him Tong, Yi Yu*, David O'Hagan and Hai Deng* Fluoroacetate biosynthesis from the marine-derived bacterium Streptomyces xinghaiensis NRRL B-24674. Org Biomol Chem, 2014, 12(27): 4828-4831.

22. Varman, A.M. #, Yu, Y. #, You L., Tang, Y.J. Photoautotrophic production of D-lactate in an engineered cyanobacterium. Microbial Cell Factories, 2013, 12: 117 (#Contributed equally).
    

    
    

23. Qin, Z., Huang, S., Yu, Y.*, Deng, H*. Dithiolopyrrolone natural products: isolation, synthesis and biosynthesis. Marine Drugs, 2013, 11(10):3970-97.

24. Yu, Y., You, L., Liu, D.Y., Hollinshead, W., Tang, Y.J., Zhang, F.Z. Development of Synechocystis sp. PCC 6803 as a Phototrophic Cell Factory. Marine Drugs, 2013, 11(8):2894-916.

25. Wang, X., Tabudravu, J., Rateb, M.E., Annand, K.J., Qin, Z., Jaspars, M., Deng, Z., Yu, Y.*, Deng, H*. Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus. Molecular Biosystems, 2013, 9(6):1286-9.

26. Wu XM, Yu Y., Han LB, Li CL, Wang HY, Zhong NQ, Yao Y, Xia GX*.The tobacco BLADE-ON-PETIOLE2 gene mediates differentiation of the corolla abscission zone by controlling longitudinal cell expansion. Plant Physiology, 2012, 159(2):835-50.

27. Zhang, Q.; Yu, Y.*, Thioether Crosslinkages Created by a Radical SAM Enzyme. ChemBioChem, 2012, 13(8), 1097-1099.

28. Huang, S.; Zhao, Y.D.; Qin, Z.W.; Wang, X.L.; Onega, M.; Chen, L.; He, J.; Yu, Y.*; Deng, H.*, Identification and heterologous expression of the biosynthetic gene cluster for holomycin produced by Streptomyces clavuligerus. Process Biochemistry, 2011, 46, 811-16.

29. Zhang, Q.; Li, Y.X.; Chen, D.D.; Yu, Y.; Duan, L.; Shen, B.; Liu, W. Radical-mediated enzymatic carbon chain fragmentation-recombination. Nature Chemical Biology, 2011, 7, 154-60.

30. Yu, Y.; Guo, H.; Zhang, Q.; Duan, L.; Ding, Y.; Liao, R.J.; Lei, C.; Shen, B.; Liu, W. NosA Catalyzing Carboxyl-Terminal Amide Formation in Nosiheptide Maturation via an Enamine Dealkylation on the Serine-Extended Precursor Peptide. J AM CHEM SOC, 2010, 132(46), 16324-26.

31. Wang, J.; Yu, Y.; Tang, K.; Liu, W.; He, X.; Huang, X.; Deng, Z. Thiopeptide antibiotic cyclothiazomycin: Identification and Analysis of its biosynthetic gene cluster in Streptomyces hygroscopicus 10-22. (2010) Appl Environ Microb, 76(7), 2335-44.

32. Ding, Y.; Yu, Y.; Pan, H.; Guo, H.; Li, Y.; Liu, W. Moving posttranslational modifications forward to biosynthesize the glycosylated thiopeptide nocathiacin I in Nocardia sp. ATCC202099. (2010) Molecular Biosystems, 6(7), 1180-85.

33. Yu, Y.; Duan, L.; Zhang, Q.; Liao, R. J.; Ding, Y.; Pan, H. X.; Wendt-Pienkowski, E.; Tang, G. L.; Shen, B.; Liu, W., Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework. (2009) ACS Chem Biol, 4(10), 855-64.

34. Liao, R. J.; Duan, L.; Lei, C.; Pan, H. X.; Ding, Y.; Zhang, Q.; Chen, D. J.; Shen, B.; Yu, Y.*; Liu, W.* Thiopeptide Biosynthesis Featuring Ribosomally Synthesized  Precursor Peptides and Conserved Posttranslational Modifications. (2009)  Chemisty & Biology, 16 (2), 141-47.

35. Liu, W.; Yu, Y. Combinatorial Biosynthesis of Pharmaceutical Natural Products. Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing (Chapter 11) Wiley Press, ISBN: 978-0-470-82314-9, Oct. 2008, 229-246.

36. Yu, Y.; Wang, H.Y.; Liu, L.N.; Chen, Z.L.; Xia, G.X. Functional identification of cytokinesis-related genes from tobacco BY-2 cells. (2007) Plant Cell Rep, 26(7), 889-94.

37. Chen, Z.L.#; Yu, Y.#; Liu, L.N.; Xia, G.X. Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells. (2007) Progress in Nature Science 17, 156-62. (#Contributed equally).

38. Yu, Y.; Bai, L. Q.; Minagawa, K.; Jian, X. H.; Li, L.; Li, J. L.; Chen, S. Y.; Cao, E. H.; Mahmud, T.; Floss, H. G.; Zhou, X. F.; Deng, Z. X., Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp jinggangensis 5008. (2005) Appl Environ Microb, 71 (9), 5066-76.

39. Wang, H.Y. #; Yu, Y. #, Chen, Z.L.; Xia, G.X. Functional characterization of Gossypium hirsutum profilin 1 gene (GhPFN1) in tobacco suspension cells. Characterization of in vivo functions of a cotton profilin gene. (2005) Planta 222, 594-603. (#Contributed equally).

40. 王岩；虞沂；赵群飞；孔毅；刘文，天然产物的生物合成和组合生物合成研究进展。国外医药（抗生素分册）2008，29（6），275-282.

41. 张华；赵群飞；虞沂；陆大年；刘文，特异性克隆编码多肽合成酶还原结构域基因的PCR方法。中国抗生素杂志 2009，34（3），189-192.

42. 吕美男；虞沂*，2-脱氧链霉胺类抗生素的生物合成研究进展。中国科技论文在线, 2014.

43. 张玉；虞沂*，吡咯酰胺类化合物合成途径中醛基转移酶Pya26的功能研究。武汉大学学报（理学版），2016，62（1）：078-084.

44. 黄胜；虞沂*，二硫吡咯酮类抗生素的生物合成研究与代谢工程应用。微生物学报（微生物药物创新专刊），2016，3.
    

专利

诺丝七肽的生物合成基因簇 (专利号：ZL 2009 1 0053427.7  授权公告日：2011年6月22日)

刘文；虞沂；段炼；雷春；丁莹；廖日晶