孙宇辉

职称/职务：二级教授、博士生导师

金沙集团wwW3354CC副院长、金沙集团wwW3354CC药物研究所所长

组合生物合成与新药发现教育部重点实验室主任

办公地址：湖北省武汉市武昌区八一路299号，武汉大学理学部测试中心大楼C-501室

办公电话：027-6875 6642

电子邮件：yhsun@whu.edu.cn

个人网址：http://yhsun.whu.edu.cn

学习背景

1988.9―1992.7    江西农业大学  攻读植物遗传育种专业学士学位

1997.9―2001.3    华中农业大学  攻读生物化学与分子生物学专业硕士学位

2001.3―2004.3    上海交通大学  攻读生物化学与分子生物学专业博士学位

工作经历

1992.7―1998.9    江西农业大学 生物技术研究开发中心，研究实习员

1998.9―2002.10   江西农业大学 生物技术研究开发中心，助理研究员

2002.10―2006.11  江西农业大学 生物工程系，副教授

2006.11―2009.11  江西农业大学 生物工程与技术学院，教授

2005.1―2010.5    剑桥大学 生物化学系，Royal Society China Fellow、Senior Research Associate

2010.5―现在     武汉大学 金沙集团wwW3354CC，教授、湖北省“楚天学者特聘教授计划”入选者

研究领域

微生物来源天然产物生物合成的分子遗传学、生物化学及合成生物学

研究方向

●微生物来源活性天然产物生物合成途径的解析与药物创新

以具有重要基础和应用价值的微生物天然产物为研究对象，从分子遗传学、生物化学及合成生物学的角度探索其生物合成机理，并回答其生物合成中蕴藏的关键科学问题，为新药创制提供精确的“分子装配线路图”。同时，通过代谢途径的理性设计和定向优化，有的放矢地改造天然产物生物合成途径，实现微生物药物的高产和创新。

● DNA编辑新技术的探究与应用

侧重于DNA编辑机制的探索和工具的开发，并综合运用DNA编辑技术对靶向基因（簇）进行高效克隆和编辑。

承担项目

科技部国家重点研发计划项目（2020-2024）

国家自然科学基金重点国际（地区）合作研究项目（2020-2024）

国家自然科学基金面上项目（2013-2016，2015-2018，2018-2021）

微生物代谢国家重点实验室（上海交通大学）开放课题（2015-2017，2018-2020）

国家重点基础研究发展计划(973)子课题（2012-2016）

国家高技术研究发展计划(863计划)子课题（2012-2015）

武汉大学医学部协同创新项目（2013-2015）

教育部海外名师项目（2012-2017）

教育部高等学校博士点专项科研基金(博导类)（2012-2014）

湖北省研究与开发计划项目（2012-2014）

国家“重大新药创制”科技重大专项子课题（2011-2013）

武汉大学自主科研项目（2011-2013）

获奖情况

湖北省第十六届自然科学优秀学术论文二等奖（2016）

湖北省第十五届自然科学优秀学术论文一等奖（2014）

武汉大学优秀烛光导航师（2014）

第六届武汉大学朱裕璧医学奖（2014）

国家自然科学二等奖（2008）

江西省高校科技成果一等奖（2007）

江西省自然科学二等奖（2006）

全国优秀博士论文提名奖（2006）

上海市研究生优秀成果（学位论文）奖（2006）

上海市科学技术进步一等奖（2004）

学术兼职

教育部高等学校生物技术、生物工程类专业教学指导委员会委员（2018年11月-2022年11月）

The Globe Industrial Microbiology Society国际委员会秘书长（2019年9月-2024年9月）

微生物代谢国家重点实验室第一届、第二届学术委员会委员（2012年1月-2021年2月）

生物催化与酶工程省部共建国家重点实验室第一届学术委员会委员（2019年11月-2023年11月）

药用植物繁育与栽培国家地方联合工程研究中心第一届学术委员会委员（2016年1月-2020年12月）

微生物分子生物学湖南省重点实验室第三届学术委员会委员（2019年5月-2024年4月）

湖北省食品药品监督管理局科技咨询专家（2016年4月-2021年4月）

中国微生物学会分子微生物学与生物工程专业委员会委员（2016年10月-2021年10月）

湖北省暨武汉微生物学会第十二届、第十三届理事会常务理事（2013年11月-2023年12月）

湖北省药学会第十二届、第十三届理事会生化制药专业委员会委员、主任委员（2013年4月-2023年4月）

《生物工程学报》第五届编辑委员会编委（2017年11月-2022年10月）

《激光生物学报》第七届编辑委员会常务编委（2018年12月）

《合成生物学》第一届编辑委员会编委（2020年1月）

《Wuhan University Journal of Natural Sciences》编委（2011年1月）

Associate Faculty Member for F1000 Prime（2014年3月27日）

教学情况

本科生《分子生物学》

硕士研究生《生物药学研究进展》

博士研究生《生物技术前沿讲座》

课题成员

现有讲师1名，博士后1名，博士研究生5名，硕士研究生9名。

已培养博士后2名，博士10名，硕士23名。

发表论文（*：通讯作者，#：第一作者）

1. Guo Sun, Chaoqun Hu, Qing Mei, Minghe Luo, Xu Chen, Zhengyuan Li, Yuanzhen Liu, Zixin Deng, Zhengyu Zhang* and Yuhui Sun*, Uncovering the cytochrome P450-catalyzed methylenedioxy bridge formation in streptovaricins biosynthesis. Nature Communications 2020, 11: 4501.

2. Guifa Zhai#, Wenyan Wang#, Wei Xu, Guo Sun, Chaoqun Hu, Xiangming Wu, Zisong Cong, Liang Deng, Yanrong Shi, Peter F. Leadlay, Heng Song, Kui Hong, Zixin Deng and Yuhui Sun*, Cross-module enoylreduction in the azalomycin F polyketide synthase. Angewandte Chemie International Edition 2020, 59: 2-7.

3. Minghe Luo, Lingjie Tang, Yulu Dong, Hongbo Huang, Zixin Deng and Yuhui Sun*, Antibacterial natural products lobophorin L and M from the marine-derived Streptomyces sp. 4506. Natural Product Research (Online ahead of print).

4. Yuanzhen Liu*, Xu Chen, Zhengyuan Li, Lixia Huang and Yuhui Sun*, Ansavaricin J, a new heterocyclic ring-fused streptovaricin from gene stvP5-deleted mutant of Streptomyces spectabilis CCTCC M2017417. Chemistry & Biodiversity 2020, 17: e1900713.

5. Yao Liu#, Yuanzhen Liu#, Zixiu Du#, Lidan Zhang, Juan Chen, Zhen Shen, Qian Liu, Juanxiu Qin, Huiying Lv, Hua Wang, Lei He, Junlan Liu, Qian Huang, Yuhui Sun*, Michael Otto* and Min Li*, Skin microbiota analysis-inspired development of novel anti-infectives. Microbiome 2020, 8: 85.

6. Sicong Li#, Qian Liu#, Zhiyu Zhong, Zixin Deng and Yuhui Sun*, Exploration of hygromycin B biosynthesis utilizing CRISPR-Cas9-associated base editing. ACS Chemical Biology 2020, 15: 1417-1423.

7. Rory Little, Fernanda C. R. Paiva, Robert Jenkins, Hui Hong, Yuhui Sun, Yuliya Demydchuk, Markiyan Samborskyy, Manuela Tosin, Finian J. Leeper, Marcio V. B. Dias and Peter F. Leadlay*, Unexpected enzyme-catalysed [4+2] cycloaddition and rearrangement in polyether antibiotic biosynthesis. Nature Catalysis 2019, 2: 1045-1054.

8. Weixin Tao*, Li Chen, Chunhua Zhao, Jing Wu, Dazhong Yan, Zixin Deng and Yuhui Sun*, In vitro packaging mediated one-step targeted cloning of natural product pathway. ACS Synthetic Biology 2019, 8: 1991-1997.

9. Zhiyu Zhong#, Junhong Guo#, Liang Deng, Li Chen, Jian Wang, Sicong Li, Wei Xu, Zixin Deng and Yuhui Sun*, Base editing in Streptomyces with Cas9-deaminase fusions. bioRxiv 2019 (doi: https://doi.org/10.1101/630137).

10. Jingjing Zhou, Chengbin Qiu, Ni Pi, Sicong Li, Xiyao Cheng, Lei Zhang, Yao Chen, Yongqi Huang*, Yuhui Sun* and Zhengding Su*, A protein biosynthesis machinery strategy for identifying P53PTC-rescuing compounds as synergic anti-tumor drugs. ChemistrySelect 2018, 3: 11048-11053.
    

    
    

11. Weixin Tao, Anna Yang, Zixin Deng and Yuhui Sun*, CRISPR/Cas9-based editing of streptomyces for discovery, characterization, and production of natural products. Frontiers in Microbiology 2018, 9: 1660.

12. Sicong Li#, Jun Zhang#, Yuanzhen Liu, Guo Sun, Zixin Deng and Yuhui Sun*, Direct genetic and enzymatic evidence for oxidative cyclization in hygromycin B biosynthesis. ACS Chemical Biology 2018, 13: 2203-2210.

13. Yuan Li#, Wan Zhang#, Hui Zhang, Wenya Tian, Lian Wu, Shuwen Wang, Mengmeng Zheng, Jinru Zhang, Chenghai Sun, Zixin Deng, Yuhui Sun, Xudong Qu* and Jiahai Zhou*, Structural basis of a broadly selective acyltransferase from the polyketide synthase of splenocin. Angewandte Chemie International Edition 2018, 57: 5823-5827.

14. Sicong Li#, Junhong Guo#, Anna Reva, Fanglu Huang, Binbin Xiong, Yuanzhen Liu, Zixin Deng, Peter F. Leadlay* and Yuhui Sun*, Methyltransferases of gentamicin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America 2018, 115:1340-1345.
    

    
    

15. Yuanzhen Liu, Xu Chen, Zhengyuan Li, Wei Xu, Weixin Tao, Jie Wu, Jian Yang, Zixin Deng and Yuhui Sun*, Functional analysis of cytochrome P450s involved in streptovaricin biosynthesis and generation of anti-MRSA analogues. ACS Chemical Biology 2017, 12: 2589-2597.

16. Priscila dos Santos Bury, Fanglu Huang, Sicong Li, Yuhui Sun, Peter F. Leadlay and Marcio Vinicius Bertacine Dias*, Structural basis of the selectivity of GenN, an aminoglycoside N-methyltransferase involved in gentamicin biosynthesis. ACS Chemical Biology 2017, 12: 2779-2787.

17. Wei Xu#, Guifa Zhai#, Yuanzhen Liu, Yuan Li, Yanrong Shi, Kui Hong, Hui Hong, Peter F. Leadlay, Zixin Deng and Yuhui Sun*, An iterative module in the azalomycin F polyketide synthase contains a switchable enoylreductase domain. Angewandte Chemie International Edition 2017, 56: 5503-5506.

18. Marie E. Yurkovich, Robert Jenkins, Yuhui Sun, Manuela Tosin* and Peter F. Leadlay*, The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase. Chemical Communications 2017, 53: 2182-2185.

19. Judith Havemann, Marie E. Yurkovich, Robert Jenkins, Sophia Harringer, Weixin Tao, Shishi Wen, Yuhui Sun, Peter F. Leadlay and Manuela Tosin*, Chemical probing of thiotetronate bio-assembly. Chemical Communications 2017, 53: 1912-1915.

20. Hui Hong, Yuhui Sun, Yongjun Zhou, Emily Stephens, Markiyan Samborskyy and Peter F. Leadlay*, Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase. Beilstein Journal of Organic Chemistry 2016, 12: 2164-2172.

21. Jianzhao Qi, Dan Wan, Hongmin Ma, Yuanzhen Liu, Rong Gong, Xudong Qu, Yuhui Sun, Zixin Deng and Wenqing Chen*, Deciphering carbamoylpolyoxamic acid biosynthesis reveals unusual acetylation cycle associated with tandem reduction and sequential hydroxylation. Cell Chemical Biology 2016, 23: 935-944.

22. Yujie Geng, Zixin Deng and Yuhui Sun*, An insight into the protospacer adjacent motif of Streptococcus pyogenes Cas9 with artificially stimulated RNA-guided-Cas9 DNA cleavage flexibility. RSC Advances 2016, 6: 33514-33522.

23. Weixin Tao#, Marie E. Yurkovich#, Shishi Wen#, Karen E. Lebe, Markiyan Samborskyy, Yuanzhen Liu, Anna Yang, Yunkun Liu, Yingchen Ju, Zixin Deng, Manuela Tosin, Yuhui Sun* and Peter F. Leadlay*, A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. Chemical Science 2016, 7: 376-385.

24. Yunkun Liu#, Weixin Tao#, Shishi Wen, Zhengyuan Li, Anna Yang, Zixin Deng and Yuhui Sun*, In vitro CRISPR/Cas9 system for efficient targeted DNA editing. mBio 2015, 6:e 01714-15.

25. Hu Zeng, Shishi Wen, Wei Xu, Zhaoren He, Guifa Zhai, Yunkun Liu, Zixin Deng and Yuhui Sun*, Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system. Applied Microbiology and Biotechnology 2015, 99: 10575-10585.

26. Chuan Huang#, Fanglu Huang#, Eileen Moison, Junhong Guo, Xinyun Jian, Xiaobo Duan, Zixin Deng, Peter F. Leadlay* and Yuhui Sun*, Delineating the biosynthesis of gentamicin X2, the common precursor of the gentamicin C antibiotic complex. Chemistry & Biology 2015, 22: 251-261.

27. Junhong Guo#, Fanglu Huang#, Chuan Huang, Xiaobo Duan, Xinyun Jian, Finian Leeper, Zixin Deng, Peter F. Leadlay* and Yuhui Sun*, Specificity and promiscuity at the branch point in gentamicin biosynthesis. Chemistry & Biology 2014, 21:608-618.

28. Yuhui Sun*, A carbonate architect emerges. Nature Chemical Biology 2014, 10:486-487. (Invited Comments)

29. Yanling Ma, Wei Xu, Jun Zhang, Sihong Zhang, Kui Hong, Zixin Deng and Yuhui Sun*, Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726. Applied Microbiology and Biotechnology 2014, 98: 8583-8590.

30. Weixin Tao#, Manghong Zhu#, Zixin Deng and Yuhui Sun*, Biosynthesis of tetronate antibiotics: A growing family of natural products with broad biological activities. Science China Chemistry 2013, 56: 1364-1371. (Invited Review)

31. Chompoonik Kanchanabanca#, Weixin Tao#, Hui Hong, Yajing Liu, Frank Hahn, Markiyan Samborskyy, Zixin Deng, Yuhui Sun* and Peter F. Leadlay*, Unusual acetylation-elimination in the formation of tetronate antibiotics. Angewandte Chemie International Edition 2013, 52: 5785-5788.

32. Marie E. Yurkovich, Petros A. Tyrakis, Hui Hong, Yuhui Sun, Markiyan Samborskyy, Kohei Kamiya and Peter F. Leadlay*, A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. ChemBioChem 2012, 13: 66-71.

33. Jeroen S. Dickschat, Olivia Vergnolle, Hui Hong, Stephen Garner, Susanna R. Bidgood, Hannah C. Dooley, Zixin Deng, Peter F. Leadlay* and Yuhui Sun*, An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis. ChemBioChem 2011, 12: 2408-2412.

    注：本论文被 “Nature Chemical Biology, 2011,7(12):856”作为“研究亮点（Research Highlights）”推荐

34. Yunlong He, Yuhui Sun, Tiangang Liu, Xiufen Zhou, Linquan Bai* and Zixin Deng, Cloning of separate meilingmycin biosynthesis gene clusters by use of acyltransferase-ketoreductase didomain PCR amplification. Applied and Environmental Microbiology 2010, 76: 3283-3292.

35. Yuhui Sun*, Frank Hahn, Yuliya Demydchuk, James Chettle, Manuela Tosin, Hiroyuki Osada and Peter F. Leadlay*, In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology 2010, 6: 99-101.注：本论文被英国Faculty of 1000收录（http://f1000.com/）并获专家正面点评。

36. Yuhui Sun, Xinyi He, Jingdan Liang, Xiufen Zhou and Zixin Deng*, Analysis of functions in plasmid pHZ1358 influencing its genetic and structural stability in Streptomyces lividans 1326. Applied Microbiology and Biotechnology 2009, 82: 303-310.

37. David H. Kwan#, Yuhui Sun#, Frank Schulz, Hui Hong, Bojana Popovic, Joalice C. C. Sim-Stark, Stephen F. Haydock and Peter F. Leadlay*, Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. Chemistry & Biology 2008, 15: 1231-1240.

38. Yuliya Demydchuk#, Yuhui Sun#, Hui Hong, James Staunton, Jonathan B. Spencer and Peter F. Leadlay*, Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. ChemBioChem 2008, 9: 1136-1145.

39. Yuhui Sun, Hui Hong, Fraser Gillies, Jonathan B. Spencer and Peter F. Leadlay*, Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics. ChemBioChem 2008, 9: 150-156.

40. Barbara M. Harvey, Tatiana Mironenko, Yuhui Sun, Hui Hong, Zixin Deng, Peter F. Leadlay, Kira J. Weissman and Stephen F. Haydock*, Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. Chemistry & Biology 2007, 14: 703-714.

41. Yuhui Sun, Hui Hong, Markiyan Samborskyy, Tatiana Mironenko, Peter F. Leadlay and Stephen F. Haydock*, Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. Microbiology 2006, 152: 3507-3515.

42. Tiangang Liu, Delin You, Chiara Valenzano, Yuhui Sun, Jialiang Li, Qing Yu, Xiufen Zhou, David E. Cane and Zixin Deng*, Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. Chemistry & Biology 2006, 13:945-955.

43. Yuhui Sun, Xiufen Zhou, Hui Dong, Guoquan Tu, Min Wang, Bofei Wang and Zixin Deng*, A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin. Chemistry & Biology 2003, 10: 431-441.

44. Yuhui Sun, Xiufen Zhou, Guoquan Tu and Zixin Deng*, Identification of a gene cluster encoding meilingmycin biosynthesis among multiple polyketide synthase contigs isolated from Streptomyces nanchangensis NS3226. Archives of Microbiology 2003, 180: 101-107.

45. Yuhui Sun, Xiufen Zhou, Jun Liu, Kai Bao, Guiming Zhang, Guoquan Tu, Tobias Kieser and Zixin Deng*, ‘Streptomyces nanchangensis’, a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters. Microbiology 2002, 148: 361-371.

46. Xiuhua Pang, Xiufen Zhou, Yuhui Sun and Zixin Deng*, Physical map of the linear chromosome of Streptomyces hygroscopicus 10-22 deduced by analysis of overlapping large chromosomal deletions. Journal of Bacteriology 2002, 184: 1958-1965.

47. Xiuhua Pang, Yuhui Sun, Liu Jun, Xiufen Zhou and Zixin Deng*, A linear plasmid temperature-sensitive for replication in Streptomyces hygroscopicus 10-22. FEMS Microbiology Letters 2002, 208: 25-28.
    

国际合作

剑桥大学生物化学系Herchel Smith讲席教授、英国皇家学会院士（FRS）、英国皇家化学会院士（FRSC）Peter F. Leadlay (http://www.bio.cam.ac.uk/~pflgroup/)

人员招聘

热忱欢迎追求完美和对科研工作富有激情，具有微生物遗传学、生物化学、化学、天然药物化学、结构生物学之一背景的博士后、研究生、本科生加入本研究组。